autoethanogenum is a strictly anaerobic, Gram-positive, spore forming, rod-like, motile bacterium. It therefore represents an extremely versatile platform for the sustainable production of commodity chemicals and fuels.Ĭ. Thus, gas fermentation allows the production of low carbon fuels and high-value chemicals without competing for food or land.
steel manufacture and oil refining, coal and natural gas ). These bacteria are capable of growth on a spectrum of waste gases from industry (e.g. One alternative solution is to develop processes based on acetogenic bacteria such as Clostridium autoethanogenum, whereby carbon is directly captured (in the form of carbon monoxide or carbon dioxide) through anaerobic gas fermentation. A range of solutions are being explored to increase the economic viability of this process, including the direct microbial conversion of biomass by lignocellulose degrading organisms. The costs involved are making the development of economic processes extremely challenging. The exploitation of biomass, however, is reliant on an energy intensive pre-treatment step, and thereafter, the addition of costly exogenous hydrolytic enzymes required to convert the partially deconstructed biomass into the sugars needed by the fermentative process organisms. To date, the focus has been on the use of lignocellulosic biomass feedstocks. One of the greatest challenges facing industry and society is the future sustainable production of chemicals and fuels from non-food resources while at the same time reducing greenhouse gas emissions.
Clc sequence viewer ncbi full#
We present a revised manually curated full genome sequence for Clostridium autoethanogenum DSM10061, which provides reliable information for genome-scale models that rely heavily on the accuracy of annotation, and represents an important step towards the manipulation and metabolic modelling of this industrially relevant acetogen. As a result, different functions were assigned to gene products or previous functional annotations rejected because of missing evidence in various occasions.
Clc sequence viewer ncbi serial#
Furthermore, we have completed a comprehensive manual curation to reduce errors in the annotation that occur through serial use of automated annotation pipelines in related species. It was also observed that many genes containing these sequencing errors were annotated in the published closed genome as encoding proteins containing frameshift mutations (18 instances) or were annotated despite the coding frame containing stop codons, which if genuine, would severely hinder the organism’s ability to survive.
This was corroborated by the observation that over 90 % occurred within homopolymer regions of greater than 4 nucleotides in length. These variations were confirmed by Sanger sequencing and subsequent analysis suggested that the discrepancies were sequencing errors in the published genome not true single nucleotide polymorphisms. We performed next generation sequencing using Illumina MiSeq technology on the DSM10061 strain of Clostridium autoethanogenum and observed 243 single nucleotide discrepancies when compared to the published finished sequence (NCBI: GCA_000484505.1), with 59.1 % present in coding regions. However, crucial to metabolic modelling and directed pathway engineering is a reliable and comprehensively annotated genome sequence. Current research efforts are focused on the enhancement and extension of product formation in this organism via synthetic biology approaches. This common industrial waste gas can act as the sole energy and carbon source for the bacterium that converts the low value gaseous components into cellular building blocks and industrially relevant products via the action of the reductive acetyl-CoA (Wood-Ljungdahl) pathway. Clostridium autoethanogenum is an acetogenic bacterium capable of producing high value commodity chemicals and biofuels from the C1 gases present in synthesis gas.
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